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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SSR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of SSR1 using rabbit SSR1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SSR1 gene (Lane 2).
Western blot analysis in Hela,mouse NIH-3T3 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SSR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

SSR1 Antibody

CATALOG NUMBER: 57-743

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human SSR1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SSR1
Additional Names:
Translocon-associated protein subunit alpha, TRAP-alpha, Signal sequence receptor subunit alpha, SSR-alpha, SSR1, TRAPA
Protein Accession Number:
P43307
Protein Gi Number:
22261821
Ncbi Gene Id Number:
6745
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are exclusively non-canonical.
Background Reference 1:
Hirama, T., et al., FEBS Lett. 455(3):223-227 (1999).
Background Reference 2:
Hartmann, E., et al., FEBS Lett. 349(3):324-326 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-743

List Size:
400 ul

List Price:

$495.00

Datasheet
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