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<strong>Figure 1 Immunoflouorescence Validation of ST2 in HeLa Cells</strong><br>Immunofluorescent analysis of PFA-fixed HeLa cells labeling ST2 with 3363 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
<strong>Figure 2 WB Validation in Human, Mouse, and Rat Cell Lines </strong><br>Loading: 15 μg of lysate per lane Antibodies: ST2 3363, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in 293 Cells </strong><br>Antibodies: ST2 3363, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: In the absence of blocking peptideLane B: In the presence of blocking peptide
<strong>Figure 4 Western Blot Validation in Mouse Heart </strong><br>Loading: 15 μg of lysateAntibodies: ST2 3363, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of ST2 in Human Lung </strong><br>Immunofluorescent analysis of PFA-fixed human lung tissue labeling ST2 with 3363 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue
<strong>Figure 6 Immunofluorescence Validation of ST2 in Human Lung Cancer Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung cancer tissue labeling ST2 with 3363 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of ST2 in Mouse Kidney </strong><br>Immunofluorescent analysis of PFA-fixed mouse kidney tissue labeling ST2 with 3363 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue)
<strong>Figure 8 Immunohistochemistry Validation of ST2 in Rat Kidney </strong><br> Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ST2 antibody (3363) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Flow Cytometry Validation of ST2 in K562 Cells 1X10^6/mL K562 cells were intracellularly stained with 2 μg/mL of ST2 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Figure 1 Immunoflouorescence Validation of ST2 in HeLa Cells
Immunofluorescent analysis of PFA-fixed HeLa cells labeling ST2 with 3363 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Cell Treatment
Trial Size

ST2 Antibody

CATALOG NUMBER: 3363

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IF, IHC-P, WB, ICC/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Horse (74%), Cow (67%)
Immunogen:
ST2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2.
This peptide is common to all three known ST2 isoforms.
The immunogen is located within amino acids 40 - 90 of ST2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF, IHC-P, WB, ICC/IF
Application Note:
WB: 1-2 μg/mL; IHC: 1-5 μg/mL; IF: 5-20 μg/mL; FC: 2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry and Immunofluorescence in human, mouse and rat samples; Flow Cytometry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 63/37 kD

Observed: 63/37 kD
Purification:
ST2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ST2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ST2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Il1rl1
Additional Names:
ST2 Antibody: T1, St2, DER4, Ly84, ST2L, Fit-1, T1/ST2, St2-rs1, Ste2, Interleukin-1 receptor-like 1, Lymphocyte antigen 84
Protein Accession Number:
BAA02854
Protein Gi Number:
286101
Ncbi Gene Id Number:
17082
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ST2 Antibody: ST2 is a member of a superfamily containing the interleukin-1 receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor (TLR) 4 signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 3:
Lanahan A, Williams JB, Sanders LK, et al. Growth factor-induced delayed early response genes. Mol Cell Biol. 1992; 12:3919-29.
Background Reference 4:
Sweet MJ, Leung BP, Kang D, et al. A novel pathway regulating lipopolysaccharide-induced shock by ST2/T1 via inhibition of Toll-like receptor 4 expression. J. Immunol. 2001; 166:6633-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3363

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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