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Antibody used in IHC on Human kidney.
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in IHC on Human kidney.

ST3GAL5 Antibody

CATALOG NUMBER: 30-197

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ST3GAL5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ST3GAL5 antibody can be used for detection of ST3GAL5 by ELISA at 1:1562500. ST3GAL5 antibody can be used for detection of ST3GAL5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
48 kDa, 46 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ST3GAL5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ST3GAL5
Additional Names:
ST3GAL5, SIAT9, SIATGM3S, ST3GalV, SATI
Protein Accession Number:
NP_003887
Protein Gi Number:
109633044
Ncbi Gene Id Number:
8869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. ST3GAL5 is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. It is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in its gene has been associated with Amish infantile epilepsy syndrome.Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus.
Background Reference 1:
Chung, T.W., (2005) Glycobiology 15 (3), 233-244.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-197

List Size:
100 ul

List Price:

$473.00

Datasheet
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