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Western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).

ST5 Antibody

CATALOG NUMBER: 62-197

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1106-1137 amino acids from the C-terminal region of human ST5.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
126 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ST5
Additional Names:
Suppression of tumorigenicity 5 protein, DENN domain-containing protein 2B, HeLa tumor suppression 1, ST5, DENND2B, HTS1
Protein Accession Number:
P78524
Protein Gi Number:
317373507
Ncbi Gene Id Number:
6764
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ST5 was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells.
Background Reference 1:
Majidi, M., et al., J. Biol. Chem. 275(9):6560-6565 (2000).
Background Reference 2:
Hubbs, A.E., et al., Oncogene 18(15):2519-2525 (1999).
Background Reference 3:
Majidi, M., et al., J. Biol. Chem. 273(26):16608-16614 (1998).
Background Reference 4:
Lichy, J.H., et al., Nucleic Acids Res. 24(23):4700-4708 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-197

List Size:
400 ul

List Price:

$495.00

Datasheet
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