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Western blot analysis of extracts of various cell lines, using ST6GAL1 antibody (19-836) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human stomach using ST6GAL1 antibody (19-836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ST6GAL1 antibody (19-836) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ST6GAL1 antibody (19-836) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ST6GAL1 Antibody

CATALOG NUMBER: 19-836

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-200 of human ST6GAL1 (NP_775323.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse thymus
Positive Control 2:
Mouse lung
Predicted Molecular Weight:
Observed: 47kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ST6GAL1
Additional Names:
SIAT1, Beta-galactoside alpha-2, 6-sialyltransferase 1, B-cell antigen CD75, Alpha 2, 6-ST 1, ST6N, ST6GalI
Ncbi Gene Id Number:
6480
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-836

List Size:
100 uL

List Price:

$436.00

Datasheet
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