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Western blot analysis of ST8SIA4 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of ST8SIA4 Antibody in mouse spleen tissue lysates (35ug/lane)
ST8SIA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ST8SIA4 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of ST8SIA4 Antibody in mouse spleen tissue lysates (35ug/lane)

ST8SIA4 Antibody

CATALOG NUMBER: 64-105

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ST8SIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ST8SIA4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ST8SIA4
Additional Names:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase, 2499-, Alpha-2,8-sialyltransferase 8D, Polysialyltransferase-1, Sialyltransferase 8D, SIAT8-D, Sialyltransferase St8Sia IV, ST8SiaIV, ST8SIA4, PST, PST1, SIAT8D
Protein Accession Number:
Q92187
Protein Gi Number:
2494834
Ncbi Gene Id Number:
7903
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus.
Background Reference 1:
Johnson, M.P., et al. Hum. Genet. 126(5):655-666(2009)
Background Reference 2:
Foley, D.A., et al. J. Biol. Chem. 284(23):15505-15516(2009)
Background Reference 3:
Otowa, T., et al. J. Hum. Genet. 54(2):122-126(2009)
Background Reference 4:
Sonuga-Barke, E.J., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (8), 1359-1368 (2008) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-105

List Size:
400 ul

List Price:

$495.00

Datasheet
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