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42-785 (0.03ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-785 (0.3ug/ml) staining of Mouse Spleen (A), Mouse Thymus (B) and Rat Spleen (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-785 (0.03ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STAG2 Antibody

CATALOG NUMBER: 42-785

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SRGSTVRSKKSKPST
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 160kDa band observed in lysates of cell line K562 and ~140kDa in rodent Spleen while ~150kDa in Mouse Thymus lysates (calculated MW of 146kDa according to Human NP_001036214.1 and 140kDa according to Mouse NP_001071180.1). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001036214.1; NP_006594.3). Reported variants NP_001036215.1 and NP_001036214.1 represent identical protein: Reported variants NP_001036216.1 and NP_006594.3 represent identical protein.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STAG2
Additional Names:
STAG2, stromal antigen 2, DKFZp686P168, DKFZp781H1753, FLJ25871, SA-2, SA2, bA517O1.1, OTTHUMP00000024338, OTTHUMP00000043514, OTTHUMP00000043515, OTTHUMP00000081754, OTTHUMP00000081755, SCC3 homolog 2
Protein Accession Number:
NP_001036214.1,NP_006594.3
Protein Gi Number:
112789526
Ncbi Gene Id Number:
10735

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-785

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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