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46-436 (0.01ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-436 (0.01ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STAG2 Antibody

CATALOG NUMBER: 46-436

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-REPKRLRPEDS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 146kDa according to NP_001036214.1 and NP_001036215.1). Recommended concentration: 0.01ug/ml.
Specificity:
This antibody is expected to recognise isoform a (NP_001036214.1 and NP_001036215.1) and isoform b (NP_001036216.1 and NP_006594.3).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STAG2
Additional Names:
STAG2, stromal antigen 2, RP11-517O1.1, DKFZp686P168, DKFZp781H1753, FLJ25871, SA-2, SA2, bA517O1.1, SCC3 homolog 2
Protein Accession Number:
NP_001036214.1,NP_001036215.1,NP_001036216.1,NP_006594.3
Protein Gi Number:
112789526
Ncbi Gene Id Number:
10735
Background Reference 1:
Lara-Pezzi E, Pezzi N, Prieto I, Barthelemy I, Carreiro C, Martinez A, Maldonado-Rodriguez A, Lopez-Cabrera M, Barbero JL. Evidence of a transcriptional co-activator function of cohesin STAG/SA/Scc3. J Biol Chem. 2004 Feb 20;279(8):6553-9. Epub 2003 Dec 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-436

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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