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46-438 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-438 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STAM2 Antibody

CATALOG NUMBER: 46-438

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-QSIDPTDSKPDSQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Spleen lysates (calculated MW of 58.2kDa according to NP_005834.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pandey et al J Biol Chem. 2000 Dec 8;275(49):38633-9; PMID: 10993906). Recommended concentration: 1-3ug/ml.
Specificity:
No cross-reaction expected with STAM1.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STAM2
Additional Names:
STAM2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 , HGNC:11358, DKFZp564C047, Hbp, STAM2A, STAM2B , STAM-like protein containing SH3 and ITAM domains 2, signal transducing adaptor molecule 2
Protein Accession Number:
NP_005834.3
Protein Gi Number:
21265031
Ncbi Gene Id Number:
10254
Background Reference 1:
Kobayashi H, Tanaka N, Asao H, Miura S, Kyuuma M, Semura K, Ishii N, Sugamura K. Hrs, a mammalian master molecule in vesicular transport and protein sorting, suppresses the degradation of ESCRT proteins signal transducing adaptor molecule 1 and 2. J Biol Chem. 2005 Mar 18;280(11):10468-77. Epub 2005 Jan 7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-438

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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