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Western blot analysis of anti-STC2 Antibody in mouse kidney tissue lysates (35ug/lane). STC2 was detected using the purified polyclonal antibody.
Stanniocalcin-2 (STC2) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Stanniocalcin-2 (STC2) Antibody for immunohistochemistry.
Western blot analysis of anti-STC2 Antibody in mouse kidney tissue lysates (35ug/lane). STC2 was detected using the purified polyclonal antibody.

Stanniocalcin-2 Antibody

CATALOG NUMBER: 57-734

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Stanniocalcin-2 (STC2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human Stanniocalcin-2 (STC2).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STC2
Additional Names:
Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2
Protein Accession Number:
O76061
Ncbi Gene Id Number:
8614
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STC2 is a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression is induced by estrogen and altered in some breast cancers.
Background Reference 1:
Ishibashi, K., et al., Biochem. Biophys. Res. Commun. 250(2):252-258 (1998).
Background Reference 2:
DiMattia, G.E., et al., Mol. Cell. Endocrinol. 146 (1-2), 137-140 (1998).
Background Reference 3:
Chang, A.C., et al., Mol. Cell. Endocrinol. 141 (1-2), 95-99 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-734

List Size:
400 ul

List Price:

$495.00

Datasheet
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