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Western blot analysis in K562 cell line lysates (35ug/lane).
STARD5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

STARD5 Antibody

CATALOG NUMBER: 56-672

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This STARD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human STARD5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STARD5
Additional Names:
StAR-related lipid transfer protein 5, START domain-containing protein 5, StARD5, STARD5
Protein Accession Number:
Q9NSY2
Protein Gi Number:
25091329
Ncbi Gene Id Number:
80765
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cholesterol homeostasis is regulated, at least in part, bysterol regulatory element (SRE)-binding proteins (e.g., SREBP1; MIM184756) and by liver X receptors (e.g., LXRA; MIM 602423). Uponsterol depletion, LXRs are inactive and SREBPs are cleaved, afterwhich they bind promoter SREs and activate genes involved incholesterol biosynthesis and uptake. Sterol transport is mediatedby vesicles or by soluble protein carriers, such as steroidogenicacute regulatory protein (STAR; MIM 600617). STAR is homologous toa family of proteins containing a 200- to 210-amino acidSTAR-related lipid transfer (START) domain, including STARD5(Soccio et al., 2002 [PubMed 12011452]).
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 3:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 4:
Chen, Y.C., et al. Am. J. Physiol. Renal Physiol. 297 (2), F380-F388 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-672

List Size:
400 ul

List Price:

$495.00

Datasheet
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