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Western blot analysis of extracts of HeLa cells, using STAT1 antibody (14-249) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody (14-249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody (14-249) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using STAT1 antibody (14-249) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody (14-249). Western blot was performed from the immunoprecipitate using STAT1 antibody (14-249) at a dilition of 1:1000.
Western blot analysis of extracts of HeLa cells, using STAT1 antibody (14-249) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

STAT1 Antibody

CATALOG NUMBER: 14-249

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 91kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STAT1
Additional Names:
STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91
Ncbi Gene Id Number:
6772
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-249

List Size:
100 uL

List Price:

$436.00

Datasheet
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