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Western blot analysis of lysed extracts from HeLa cells using STAT3 (Ab-705).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Ab-705).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Ab-705) antibody (#21045, Red).
Western blot analysis of lysed extracts from HeLa cells using STAT3 (Ab-705).

STAT3 (Ab 705) Antibody

CATALOG NUMBER: 79-286

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
STAT3 (Ab-705) antibody was raised against a peptide sequence around aa.703~707 (A-P-Y-L-K) derived from Human STAT3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: IF:1:100~1:200
Specificity:
This antibody detects endogenous level of total STAT3 protein.
Predicted Molecular Weight:
88 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
STAT3
Additional Names:
APRF, HIES, BSP1, MADH1, MADR1, APRF, Acute-phase response factor
Protein Accession Number:
NP_003141.2
Protein Gi Number:
21618338
Ncbi Gene Id Number:
6774
Background:
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Background Reference 1:
Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804. Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115. Garcia R, et al. (1998) J Biomed Sci. 5: 79-85. Bromberg J F, et al. (1999) Cell. 98: 295-303.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-286

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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