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45-153 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-153 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-153 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STAT3 Antibody

CATALOG NUMBER: 45-153

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: CRPESQEHPEADP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 90kDa band observed in Human Heart lysates (calculated MW of 88.1kDa according to NP_644805.1 and of NP_003141.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STAT3
Additional Names:
STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3
Protein Accession Number:
NP_644805.1,NP_003141.2,NP_998827.1
Protein Gi Number:
21618340
Ncbi Gene Id Number:
6774
Background Reference 1:
Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-153

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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