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Western blot analysis of extracts of NCI-H460 cells, using STAT4 antibody (22-613) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectum using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using STAT4 antibody (22-613). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of NCI-H460 cells, using STAT4 antibody (22-613) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

STAT4 Antibody

CATALOG NUMBER: 22-613

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
NCI-H460
Predicted Molecular Weight:
Observed: 76kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STAT4
Additional Names:
STAT4, SLEB11
Ncbi Gene Id Number:
6775
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-613

List Size:
100 uL

List Price:

$436.00

Out of stock

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