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Western blot analysis in HL-60,MCF-7,mouse NIH/3T3 cell line, rat liver and spleen lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in HL-60,MCF-7,mouse NIH/3T3 cell line, rat liver and spleen lysates (35ug/lane).

STAT5b Antibody

CATALOG NUMBER: 58-172

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This STAT5b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 760-787 amino acids from the C-terminal region of human STAT5b.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
90 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STAT5B
Additional Names:
Signal transducer and activator of transcription 5B, STAT5B
Protein Accession Number:
P51692
Protein Gi Number:
41019536
Ncbi Gene Id Number:
6777
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STAT5b is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.
Background Reference 1:
Xi, S., et al., Cancer Res. 63(20):6763-6771 (2003).
Background Reference 2:
Uddin, S., et al., Biochem. Biophys. Res. Commun. 308(2):325-330 (2003).
Background Reference 3:
Zhang, X., et al., J. Allergy Clin. Immunol. 112(1):93-101 (2003).
Background Reference 4:
Yamashita, H., et al., Oncogene 22(11):1638-1652 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-172

List Size:
400 ul

List Price:

$495.00

Datasheet
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