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<strong>WB Validation in Mouse Brain</strong><br>Loading: 10 μg of lysate per lane.Antibodies: Steap1 4305, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 secLane 1: 0.5 μg/mLLane 2: 1 μg/mL
<strong> WB Validation in Rat Brain</strong><br>Loading: 10 μg of lysate per lane.Antibodies: Steap1 4305, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 secLane 1: 0.5 μg/mLLane 2: 1 μg/mL
Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 μg/mL.
Immunofluorescence of STEAP1 in human spleen tissue with STEAP1 antibody at 20 μg/mL.
Flow Cytometry Validation of STEAP1 in A549 Cells 1X10^6/mL A549 cells were intracellularly stained with 2 μg/mL of STEAP1 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
WB Validation in Mouse Brain
Loading: 10 μg of lysate per lane.Antibodies: Steap1 4305, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 secLane 1: 0.5 μg/mLLane 2: 1 μg/mL
Trial Size

STEAP1 Antibody

CATALOG NUMBER: 4305

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IHC-P, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%)
Immunogen:
STEAP1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human STEAP1.
The immunogen is located within amino acids 130 - 180 of STEAP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, WB, IHC-P/IF
Application Note:
WB: 1-2 μg/mL; IHC-P: 2.5 μg/mL; IHC-P/IF: 20 μg/mL; FC: 2 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry, Immunofluorescence and Flow Cytometry in human samples. All other applications and species not yet tested.
Specificity:
This STEAP1 antibody does not cross-react with other STEAP proteins.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
STEAP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
STEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
STEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STEAP1
Additional Names:
STEAP1 Antibody: STEAP, PRSS24, STEAP, Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1
Protein Accession Number:
EAL24166
Protein Gi Number:
51094921
Ncbi Gene Id Number:
26872
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STEAP1 Antibody: The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.
Background Reference 1:
Hubert RS, Vivanco I, Chen E, et al. STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc. Natl. Acad. Sci. USA1999; 96:14523-8.
Background Reference 2:
Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
Background Reference 3:
Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9.
Background Reference 4:
Alves PM, Faure O, Graff-Dubois S, et al. STEAP, a prostate tumor antigen, is a target of human CD8+ T cells. Cancer Immunol. Immunother.2006; 55:1515-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4305

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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