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Western blot analysis of STEAP2 in human prostate tissue lysate with STEAP2 antibody at 1 μg/mL.
Immunohistochemistry of STEAP2 in human colon tissue with STEAP2 antibody at 2.5 μg/mL.
Immunofluorescence of STEAP2 in Human Colon cells with STEAP2 antibody at 20 μg/mL.
Western blot analysis of STEAP2 in human prostate tissue lysate with STEAP2 antibody at 1 μg/mL.
Trial Size

STEAP2 Antibody

CATALOG NUMBER: 4307

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
STEAP2 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human STEAP2.
The immunogen is located within the last 50 amino acids of STEAP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
STEAP2 antibody can be used for detection of STEAP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This STEAP2 antibody does not cross-react with other STEAP proteins.
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 55 kDa
Purification:
STEAP2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
STEAP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
STEAP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STEAP2
Additional Names:
STEAP2 Antibody: STMP, IPCA1, PUMPCn, STAMP1, PCANAP1, UNQ6507/PRO23203, Metalloreductase STEAP2, Prostate cancer-associated protein 1
Protein Accession Number:
Q8NFT2
Protein Gi Number:
83286886
Ncbi Gene Id Number:
261729
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STEAP2 Antibody: The six-transmembrane epithelial antigen of prostate 2 (STEAP2) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 3 and STEAP4), STEAP2 promotes both iron and copper reduction. STEAP2 expression in transfected cells also correlated with iron or copper uptake, suggesting that the STEAP family of proteins may function to stimulate iron and copper uptake. STEAP2 is widely expressed in many tissues in the plasma membrane, but is most highly expressed in prostate. At least three isoforms of STEAP2 are known to exist.
Background Reference 1:
Porkka KP, Helenius MA and Visakorpi T. Cloning and characterization of a novel six-transmembrane protein STEAP2, expressed in normal and malignant prostate. Lab Invest.2002; 82:1573-82.
Background Reference 2:
Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
Background Reference 3:
Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4307

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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