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Western blot analysis of STEAP3 in HeLa cell lysate with STEAP3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of STEAP3 in mouse liver tissue with STEAP3 antibody at 2.5 μg/mL.
Immunofluorescence of STEAP3 in Mouse Liver cells with STEAP3 antibody at 20 μg/mL.
Western blot analysis of STEAP3 in HeLa cell lysate with STEAP3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

STEAP3 Antibody

CATALOG NUMBER: 4311

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
STEAP3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human STEAP3.
The immunogen is located within the first 50 amino acids of STEAP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
STEAP3 antibody can be used for detection of STEAP3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
This STEAP3 antibody does not cross-react with other STEAP proteins.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 55 kDa
Observed: 51 kDa
Purification:
STEAP3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
STEAP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
STEAP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STEAP3
Additional Names:
STEAP3 Antibody: STMP3, TSAP6, AHMIO2, dudlin-2, Metalloreductase STEAP3, Dudulin-2, hTSAP6
Protein Accession Number:
AAH95421
Protein Gi Number:
127801437
Ncbi Gene Id Number:
55240
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STEAP3 Antibody: The six-transmembrane epithelial antigen of prostate 3 (STEAP3) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 2 and STEAP4), STEAP3 promotes both iron and copper reduction. STEAP3 is highly expressed in hematopoietic tissues and colocalizes with the transferrin endosome. Overexpression of STEAP3 stimulates iron reduction; mice lacking STEAP3 are deficient in erythroid ferrireductase activity, suggesting that STEAP3 is an endosomal ferrireductase required for transferrin-dependent iron uptake in erythroid cells.
Background Reference 1:
Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9.
Background Reference 2:
Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
Background Reference 3:
Graham RM, Chua ACG, Herbison CE, et al. Liver iron transport. World J. Gastroenterol.2007; 13:4725-36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4311

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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