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Western blot analysis of Stella in 293 cell lysate with Stella antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of Stella in 293 cell lysate with Stella antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Stella Antibody

CATALOG NUMBER: 4755

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Stella antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Stella.
The immunogen is located within the last 50 amino acids of Stella.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Stella antibody can be used for detection of Stella by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Purification:
Stella Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Stella Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Stella antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPPA3
Additional Names:
Stella Antibody: STELLA, STELLAR, Developmental pluripotency-associated protein 3, Stella-related protein
Protein Accession Number:
NP_954980
Protein Gi Number:
40548326
Ncbi Gene Id Number:
359787
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Stella Antibody: Stella was initially identified in primordial germ cells and pre-implantation embryos whose expression as a maternal factor is important in early embryonic development but is not required for germ cell specification in mice. In humans, Stella is thought to be a marker for pluripotency in embryonic stem (ES) cells as its expression is observed in primordial germ cells of both sexes and germ cell tumors but not in normal somatic tissues. However, in ES cell colonies, heterogeneous expression of Stella was seen in high throughput in situ hybridization assays, indicating that higher levels of complexity exist in otherwise thought to be undifferentiated ES cells. At least two distinct isoforms of Stella are known to exist.
Background Reference 1:
Sato M, Kimura T, Kurokawa K, et al. Identification of PGC7, a new gene expressed specifically in preimplatation embryos and germ cells. Mech. Dev.2002; 113:91-4.
Background Reference 2:
Bortvin A, Goodheart M, Liao M, et al. Dppa3/Pgc7/stella is a maternal factor and is not required for germ cell specification in mice. BMC Dev. Biol.2004; 4:2.
Background Reference 3:
Bowles J, Teasdale RP, James K, et al. Dppa3 is a marker of pluripotency and has a human homologue that is expressed in germ cell tumours. Cytogenet. Genome Res.2003; 101:261-5.
Background Reference 4:
Carter MG, Stagg CA, Falco G, et al. An in situ hybridization-based screen for heterogeneously expressed genes in mouse ES cells. Gene Expr. Patterns2008; 8:181-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4755

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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