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Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.
Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 μg/ml.
Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 μg/mL.<br><br>Green: STIM1 Antibody (4119) <br> Blue: DAPI staining
Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 μg/mL.<br><br>Red: STIM1 Antibody (4119) <br> Blue: DAPI staining
Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

STIM1 Antibody

CATALOG NUMBER: 4119

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.
The immunogen is located within the last 50 amino acids of STIM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
Positive Control 1:
Cat. No. 1409 - Mouse Thymus Tissue Lysate
Positive Control 2:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 3:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 59, 75, 87 kDa
Observed: 88 kDa
Purification:
STIM1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
STIM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STIM1
Additional Names:
STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, Stromal interaction molecule 1
Protein Accession Number:
NP_003147
Protein Gi Number:
21070997
Ncbi Gene Id Number:
6786
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STIM1 Antibody: In T lymphocytes, the sole pathway for Ca2+ entry following antigen-receptor binding is through store-operated Ca2+-release-activated Ca2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca2+ entry.
Background Reference 1:
Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
Background Reference 2:
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
Background Reference 3:
Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 2005; 437:902-5.
Background Reference 4:
Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4119

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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