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Western blot analysis of extracts of various cell lines, using STIP1 antibody (15-375) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using STIP1 antibody (15-375). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using STIP1 antibody (15-375) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

STIP1 Antibody

CATALOG NUMBER: 15-375

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human STIP1 (NP_006810.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
COS-7
Positive Control 3:
COS-1
Positive Control 4:
OVCAR3
Positive Control 5:
Mouse brain
Predicted Molecular Weight:
Observed: 69kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STIP1
Additional Names:
STIP1, HOP, IEF-SSP-3521, P60, STI1, STI1L, HEL-S-94n
Ncbi Gene Id Number:
10963
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-375

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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