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42-982 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-982 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STK3 Antibody

CATALOG NUMBER: 42-982

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DFKNKSHENCNQN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 65kDa band observed in lysates of cell lines A431 and NIH3T3 (calculated MW of 56.3kDa according to Human NP_006272.2 and 56.8kDa according to Mouse NP_062609.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is not expected to cross-react to STK4
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
Approx 65 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STK3
Additional Names:
STK3, serine/threonine kinase 3 (STE20 homolog, yeast), FLJ90748, KRS1, MST2, serine/threonine kinase 3 (Ste20, yeast homolog)
Protein Accession Number:
NP_006272.2
Protein Gi Number:
103471999
Ncbi Gene Id Number:
6788

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-982

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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