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46-445 (2ug/ml) staining of nuclear HEK293 (A) and (1ug/ml) Human Breast cancer (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-445 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
46-445 (1ug/ml) staining of Mouse (A) and (2ug/ml) Pig (B) Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-445 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
46-445 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
46-445 (2ug/ml) staining of nuclear HEK293 (A) and (1ug/ml) Human Breast cancer (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

STK35 Antibody

CATALOG NUMBER: 46-445

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: ELETRMDQVTCAA
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1kDa according to Human NP_543026.2, 59.5kDa according to Mouse NP_899085.3 and 59.2kDa according to XP_005672850.1 Pig XP_005672850.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: Goyal P et al, PLoS One. 2009 Sep 16;4(9):e6981. PMID: 19756140.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STK35
Additional Names:
STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
Protein Accession Number:
NP_543026.2
Protein Gi Number:
20128768
Ncbi Gene Id Number:
140901
Background Reference 1:
Vallenius T, Makela TP. Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. J Cell Sci. 2002 May 15;115(Pt 10):2067-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-445

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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