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Western blot analysis of Stk39 in SK-N-SH cell lysate with Stk39 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Stk39 in human brain tissue with Stk39 antibody at 2.5 μg/mL.
Immunofluorescence of stk39 in human brain tissue with stk39 antibody at 20 μg/mL.
Western blot analysis of Stk39 in SK-N-SH cell lysate with Stk39 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Stk39 Antibody

CATALOG NUMBER: 4855

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Pig: (95%)
Immunogen:
Stk39 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human Stk39.
The immunogen is located within amino acids 450 - 500 of Stk39.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Stk39 antibody can be used for detection of stk39 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
Stk39 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Stk39 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Stk39 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STK39
Additional Names:
Stk39 Antibody: DCHT, PASK, SPAK, DCHT, Ste-20-related kinase
Protein Accession Number:
NP_037365
Protein Gi Number:
115430252
Ncbi Gene Id Number:
27347
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Stk39 Antibody: The serine/threonine kinase Stk39 belongs to the STE20 family, a group of kinases that are known to interact with inflammation-related kinases (such as p38, JNK, NKCC1, PKC-theta, WNK and MLCK), and with transcription factor AP-1. The STE 20 family is involved in diverse biological phenomena, including cell differentiation, cell transformation/ proliferation, cytoskeleton rearrangement, and the regulation of ion transporters. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain and is abundantly expressed in the brain. STK39 is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled co-transporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. Recent studies show that STK39 tend to be a novel candidate gene for autism and hypertension.
Background Reference 1:
Johnston AM, Nacelli G, Gonzales LJ, et al. SPAK, a STE20/SPS1-related kinase that activates the p38 pathway. Oncogene2000; 19:4290-7.
Background Reference 2:
Li Y, Hu J, Vita R, et al. SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway. EMBO J.2004; 23:1112-22.
Background Reference 3:
Gagnon KB, England R, and Delpire E. Characterization of SPAK and OSR1, regulatory kinases of the Na-K-2Cl cotransporter. Mol. Cell Biol.2006; 26:689-98.
Background Reference 4:
Dan I, Watanabe NM, and Kasumi A. The Ste20 group kinases as regulators of MAP kinase cascades. Trends Cell. Biol.2001; 11:220-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4855

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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