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Western blot analysis of extracts of various cells, using STK4 antibody (23-365) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody (23-365) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody (23-365) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug STK4 antibody (23-365). Western blot was performed from the immunoprecipitate using STK4 antibody (23-365) at a dilition of 1:1000.
Western blot analysis of extracts of various cells, using STK4 antibody (23-365) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

STK4 Antibody

CATALOG NUMBER: 23-365

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human STK4 (NP_006273.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
U-87MG
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
MCF-7
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STK4
Additional Names:
Serine/threonine-protein kinase 4, Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2, Serine/threonine-protein kinase 4 37kDa subunit, MST1/N, Serine/threonine-protein kinase 4 18kDa subunit, MST1/C, STK4, KRS2, MST1
Ncbi Gene Id Number:
6789
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-365

List Size:
100 uL

List Price:

$540.00

Datasheet
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