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46-715 (1.7ug/ml) staining of 3T3-L1 cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-715 (1ug/ml) staining of Rat Testes lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-715 (1.7ug/ml) staining of 3T3-L1 cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

STRA6 Antibody

CATALOG NUMBER: 46-715

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-QAFRKAALTSAKAN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-90kDa band observed in Rat Testis lysates, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 73.8kDa according to Rat NP_001025095.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.

An approx. 90kDa band was also observed in 3T3-L1 adipocytes reduced by stra6 siRNA. The 90kDa is stained after retrovirally overexpressing Mouse Stra6 and not after overexpressing Human Stra6 in 3T3 preadipocytes (calculated MW of 73.8kDa according to Mouse NP_033317.2). Primary incubation was 3 hours.

Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Stra6
Additional Names:
STRA6, stimulated by retinoic acid gene 6 homolog (mouse) , FLJ12541, MCOPS9, PP14296 , stimulated by retinoic acid gene 6 homolog
Protein Accession Number:
NP_033317.2,NP_001025095.1
Protein Gi Number:
6678171
Ncbi Gene Id Number:
20897(mouse);363071(rat);
Background Reference 1:
Kawaguchi R, Yu J, Honda J, Hu J, Whitelegge J, Ping P, Wiita P, Bok D, Sun H. A membrane receptor for retinol binding protein mediates cellular uptake of vitamin A. Science. 2007 Feb 9;315(5813):820-5. Epub 2007 Jan 25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-715

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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