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Western blot analysis of STRAP Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with STRAP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of hela cells using STRAP Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of STRAP using rabbit polyclonal STRAP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STRAP gene.
Western blot analysis of STRAP Antibody in Hela cell line lysates (35ug/lane)

STRAP Antibody

CATALOG NUMBER: 61-610

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This STRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human STRAP.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STRAP
Additional Names:
Serine-threonine kinase receptor-associated protein, MAP activator with WD repeats, UNR-interacting protein, WD-40 repeat protein PT-WD, STRAP, MAWD, UNRIP
Protein Accession Number:
Q9Y3F4
Protein Gi Number:
12643951
Ncbi Gene Id Number:
11171
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the SMN complex.
Background Reference 1:
Adams,C.J., et.al., EMBO Rep.(12), 1222-1229 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-610

List Size:
400 ul

List Price:

$495.00

Datasheet
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