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<strong>Figure 1 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br>Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein.Antibodies: StrepII-tag 4337 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br>Loading: 30 ng of StrepII-tagged recombinant protein per lane.Antibodies: StrepII-tag 4337, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μg/mLLane 2: 0.5 μg/mLLane 3: 1 μg/mL
Figure 1 Western Blot Validation with StrepII-tagged Recombinant Protein
Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein.Antibodies: StrepII-tag 4337 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

StrepII-tag Antibody

CATALOG NUMBER: 4337

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Tag
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Tag
Immunogen:
Anti-StrepII-tag antibody (4337) StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga".
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.25-1 μg/mL
Antibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins
Validation:

Recombinant Protein Test (Figure 1&2): Anti-StrepII-tag antibodies (4337) detected StrepII-tagged recombinant protein at different concentrations

Purification:
StrepII-tag Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
StrepII-tag antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Additional Names:
StrepII-tag Antibody:
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
Background Reference 1:
Schmidt and SKerra. Protein Eng.1993;6:109-22.
Background Reference 2:
Schmidt et al. J. Mol. Biol. 1996;255:753-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4337

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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