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Immobilized Nivolumab at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Avitag,His Tag with a linear range of 0.8-13 ng/mL when detected by Streptavidin-HRP (Routinely tested).
Serial dilutions of SARS-CoV-2 inhibitor (1:2 serial dilutions, from 20 ug/mL to 0.039 ug/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions detected by Streptavidin-HRP (Routinely test)
Immobilized Nivolumab at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Avitag,His Tag with a linear range of 0.8-13 ng/mL when detected by Streptavidin-HRP (Routinely tested).

Streptavidin-HRP Recombinant Protein

CATALOG NUMBER: 11-221

Tested Applications:
ELISA, WB
Specifications
Source Species:
E. coli
Source Species:
E. coli
Recombinant Protein Sequence:
N/A
Fusion Tag:
N/A
Tested Applications:
ELISA, WB
Application Note:
N/A
Predicted Molecular Weight:
N/A
Purity:
N/A
Physical State:
Lyophilized
Buffer:
Na3PO4 and NaCl pH6.8
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Names:
Streptavidin,SA
Background:
Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
Background Reference 1:
Green, NM., 1975, Advances in protein chemistry 29: 85–133.
Background Reference 2:
Hendrickson, W. A., 1989, Proceedings of the National Academy of Sciences 86 (7): 2190–4.
Background Reference 3:
Chivers Claire E., et al., 2010, Nature Methods 7 (5): 391–3.
Background Reference 4:
Osojic GN., et al., 2012, Small 8 (12): 1840–5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-221

List Size:
1 mg

List Price:

$350.00

Out of stock

Datasheet
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