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Streptococcus pyogenes IdeS, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Streptococcus pyogenes IdeS, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Streptococcus pyogenes IdeS Recombinant Protein

CATALOG NUMBER: 11-129

Tested Applications:
WB
Specifications
source-species:
E. coli
Species:
Streptococcus pyogenes
source-species:
E. coli
Recombinant Protein Sequence:
Asp 30 - Asn 339
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).
Predicted Molecular Weight:
36.8 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Liquid
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Names:
IgG-degrading protease,Sib38,IdeS
Protein Accession Number:
WP_010922160.1
Background:
IdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG. The bacterial protein Mac-1 adopts an alpha/beta fold, with 14 beta strands and 9 alpha helices. The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets. Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab‘)2 and Fc/2 fragments. IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species. IdeS is cloned from Streptococcus pyogenes and expressed in E. coli.
Background Reference 1:
Wenig K, Chatwell L, et al. Proc. Natl. Acad. Sci. U.S.A. 101, 17371-6, (2004).
Background Reference 2:
Hulting G, Flock M, et al. FEMS Microbiol. Lett. 298, 44-50, (2009).
Background Reference 3:
Seele J, Singpiel A, et al. J. Bacteriol. 195, 930-40, (2013).

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-129

List Size:
2500U

List Price:

$432.00

Datasheet
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