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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

STUB1 Antibody

CATALOG NUMBER: 56-259

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This STUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the C-terminal region of human STUB1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STUB1
Additional Names:
E3 ubiquitin-protein ligase CHIP, 632-, Antigen NY-CO-7, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1 {ECO:0000312, HGNC:HGNC:11427}, STUB1 (HGNC:11427)
Protein Accession Number:
Q9UNE7
Protein Gi Number:
78099173
Ncbi Gene Id Number:
10273
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STUB1, or CHIP, is a ubiquitin ligase/cochaperone thatparticipates in protein quality control by targeting a broad rangeof chaperone protein substrates for degradation (Min et al., 2008[PubMed 18411298]).
Background Reference 1:
Stankiewicz, M., et al. FEBS J. 277(16):3353-3367(2010)
Background Reference 2:
Naito, A.T., et al. Circ. Res. 106(11):1692-1702(2010)
Background Reference 3:
Loffek, S., et al. Hum. Mutat. 31(4):466-476(2010)
Background Reference 4:
Graf, C., et al. Biochemistry 49(10):2121-2129(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-259

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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