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Western blot analysis of STX7 Antibody in MDA-MB231 cell line lysates (35ug/lane)
STX7 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of STX7 Antibody in MDA-MB231 cell line lysates (35ug/lane)

STX7 Antibody

CATALOG NUMBER: 62-003

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This STX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-73 amino acids from the N-terminal region of human STX7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STX7
Additional Names:
Syntaxin-7, STX7
Protein Accession Number:
O15400
Ncbi Gene Id Number:
8417
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.
Background Reference 1:
Prekeris, R., et al. Mol. Biol. Cell 10(11):3891-3908(1999)
Background Reference 2:
Steegmaier, M., et al. J. Biol. Chem. 273(51):34171-34179(1998)
Background Reference 3:
Wong, S.H., et al. J. Biol. Chem. 273(1):375-380(1998)
Background Reference 4:
Wang, H., et al. Gene 199 (1-2), 39-48 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-003

List Size:
400 ul

List Price:

$495.00

Datasheet
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