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Western blot analysis of extracts of various cell lines, using STXBP1 antibody (19-651) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using STXBP1 antibody (19-651) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

STXBP1 Antibody

CATALOG NUMBER: 19-651

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat, Zebrafish
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K-562
Positive Control 3:
Mouse lung
Positive Control 4:
Mouse brain
Predicted Molecular Weight:
Observed: 68kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STXBP1
Additional Names:
STXBP1, MUNC18-1, UNC18, hUNC18, rbSec1, P67, NSEC1, RBSEC1
Ncbi Gene Id Number:
6812
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-651

List Size:
100 uL

List Price:

$436.00

Datasheet
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