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Western blot analysis using STYK1 monoclonal antibody against truncated STYK1 - His recombinant protein and STYK1 (aa47 - 422) / hIgGFc transfected CHO - K1 cell lysate.
Immunohistochemical analysis of paraffin - embedded human ovary carcinoma (top) and human normal cerebrum (bottom) showing cytoplasmic location using STYK1 / NOK antibody with DAB staining.
Western blot analysis using STYK1 monoclonal antibody against truncated STYK1 - His recombinant protein and STYK1 (aa47 - 422) / hIgGFc transfected CHO - K1 cell lysate.

STYK1 Antibody [2H2F10]

CATALOG NUMBER: 32-221

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant STYK1-His is expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
STYK1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STYK1
Additional Names:
NOK, Novel oncogene with kinase domainNOK, SuRTK106
Protein Accession Number:
Q6J9G0
Protein Gi Number:
317373546
Ncbi Gene Id Number:
55359
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinases (PKs) represent a well studied but most diverse protein superfamily. The covalent, reversible linkage of phosphate to serine, threonine, and tyrosine residues of substrate proteins by protein kinases is probably ubiquitous cellular mechanism for regulation of physiological processes. It is known to us that most signaling pathways impinge at some point on protein kinases. Here we report a human putative receptor protein kinase cDNA STYK1. The STYK1 cDNA is 2749 base pairs in length and contains an open reading frame encoding 422 amino acids. The STYK1 gene is mapped to human chromosome 12p13 and 11 exons were found. RT-PCR showed that STYK1 is widely expressed in human tissues.
Background Reference 1:
Liu L, Yu XZ and Li TS, et al. Mol Biol Rep. 2003, Jun, 30(2):91-6.
Background Reference 2:
Moriai R. , Kobayashi D.and Amachika T. , et al. Mol Biol Rep. 2007, Apr, 6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-221

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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