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Western blot analysis of extracts of various cell lines, using SUCLG1 antibody (16-002) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using SUCLG1 antibody (16-002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

SUCLG1 Antibody

CATALOG NUMBER: 16-002

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 41-346 of human SUCLG1 (NP_003840.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
HepG2
Positive Control 3:
A-549
Positive Control 4:
Mouse thymus
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 26-36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SUCLG1
Additional Names:
GALPHA, MTDPS9, SUCLA1, succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial, SCS-alpha, succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial, succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial, succinyl-CoA synthetase subunit alpha
Ncbi Gene Id Number:
8802
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-002

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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