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Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (23-768) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using SUCLG2 Polyclonal Antibody (23-768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SUCLG2 Polyclonal Antibody (23-768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (23-768) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

SUCLG2 Antibody

CATALOG NUMBER: 23-768

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
HT-29
Positive Control 2:
293T
Positive Control 3:
LO2
Positive Control 4:
U-87MG
Positive Control 5:
MCF7
Positive Control 6:
Raji
Predicted Molecular Weight:
Observed: 47kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SUCLG2
Additional Names:
G-SCS, GBETA, GTPSCS, succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial, GTP-specific succinyl-CoA synthetase beta subunit, GTP-specific succinyl-CoA synthetase subunit beta, SCS-betaG, succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial, succinyl-CoA ligase, GDP-forming, beta chain, mitochondrial, succinyl-CoA synthetase, beta-G chain
Ncbi Gene Id Number:
8801
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-768

List Size:
100 uL

List Price:

$540.00

Datasheet
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