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Western blot analysis of SUMO2/3 in Jurkat cell lysate with SUMO2/3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.
Immunofluorescence of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 20 μg/mL.
Western blot analysis of SUMO2/3 in Jurkat cell lysate with SUMO2/3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SUMO2/3 Antibody

CATALOG NUMBER: 5755

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (93%), Pig: (93%)
Immunogen:
SUMO2/3 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SUMO2.
The immunogen is located within the last 50 amino acids of SUMO2/3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SUMO2/3 antibody can be used for detection of SUMO2 and SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Predicted: 15 kDa
Observed: 15 kDa
Purification:
SUMO2/3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SUMO2/3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SUMO2/3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SUMO2
Additional Names:
SUMO2/3 Antibody: HSMT3, SMT3B, SUMO3, Smt3A, SMT3H2, SMT3A, Small ubiquitin-related modifier 2, HSMT3, SUMO-2
Protein Accession Number:
AAH71645
Protein Gi Number:
47940079
Ncbi Gene Id Number:
6613
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SUMO2/3 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.
Background Reference 1:
Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
Background Reference 2:
Kim KI, Baek SH, and Chung CH. Versatile protein tag, SUMO: its enzymology and biological function. J. Cell. Physiol.2002; 191: 257-68.
Background Reference 3:
Su H and Li SS. Molecular features of human ubiquitin-like SUMO genes and their encoded proteins. Gene2002; 296: 65.
Background Reference 4:
Saitoh H and Hinchey J. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3. J. Biol. Chem.2000; 275:6252-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5755

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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