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Western blot analysis using survivin monoclonal antibody against full - length survivin - His recombinant protein and full - length survivin / GFP transfected COS - 7 cell lysate.
Western blot analysis using survivin monoclonal antibody against full - length survivin - His recombinant protein and full - length survivin / GFP transfected COS - 7 cell lysate.

Survivin Antibody [2H5H2]

CATALOG NUMBER: 32-222

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified full-length recombinant Survivin-His is expressed in E.Coli strain BL21 (DE3).The antigen is soluble.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Survivin monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BIRC5
Additional Names:
API4, IAP4, Apoptosis inhibitor 4API4, EPR-1
Protein Accession Number:
O15392
Protein Gi Number:
380865472
Ncbi Gene Id Number:
332
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Baculoviral IAP repeat-containing 5 (survivin).This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
Background Reference 1:
Urology. 2007 Sep;70(3):482-6.
Background Reference 2:
World J Gastroenterol. 2007 May 28;13(20):2784-90.
Background Reference 3:
Histopathology. 2007 Jun;50(7):835-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-222

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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