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Western blot analysis of lysates from HepG2, HUVEC cell line (from left to right), using SUV39H2 Antibody (K48) at 1:1000 at each lane.
Antibody is used in Western blot to detect SUV39H2 in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of lysates from HepG2, HUVEC cell line (from left to right), using SUV39H2 Antibody (K48) at 1:1000 at each lane.

SUV39H2 Antibody

CATALOG NUMBER: 56-669

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This SUV39H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of human SUV39H2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SUV39H2
Additional Names:
Histone-lysine N-methyltransferase SUV39H2, Histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, Lysine N-methyltransferase 1B, Suppressor of variegation 3-9 homolog 2, Su(var)3-9 homolog 2, SUV39H2, KMT1B
Protein Accession Number:
Q9H5I1
Protein Gi Number:
25091325
Ncbi Gene Id Number:
79723
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The murine gene Suv39h2 encodes an H3 histone methyltransferase (HMTase) 59% identical in sequence to mouse Suv39h1. During embryogenesis, both proteins overlap in tissue expression, yet Suv39h2 transcripts are restricted to the testes in adult animals. Immunolocalization of the Suv39h2 protein during spermatogenesis indicates enrichment at the heterochromatin from the leptotene to the round spermatid stage. Moreover, Suv39h2 specifically accumulates with chromatin of the sex chromosomes, which undergo transcriptional silencing during the first meiotic prophase. Suv39h2 HMTase may also organize meiotic heterochromatin with the potential for epigenetic imprint to the male germline.
Background Reference 1:
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-669

List Size:
400 ul

List Price:

$495.00

Datasheet
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