Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of SUZ12 in human liver tissue lysate with SUZ12 antibody at 1 μg/ml.
Immunohistochemistry of SUZ12 in human lung tissue with SUZ12 antibody at 5 μg/mL.
Immunofluorescence of SUZ12 in human lung tissue with SUZ12 antibody at 20 μg/mL.
Western blot analysis of SUZ12 in human liver tissue lysate with SUZ12 antibody at 1 μg/ml.
Trial Size

SUZ12 Antibody

CATALOG NUMBER: 7701

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (79%)
Immunogen:
SUZ12 antibody was raised against a 19 amino acid peptide near the center of human SUZ12.
The immunogen is located within amino acids 400 - 450 of SUZ12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SUZ12 antibody is human specific.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 81 kDa
Observed: 96 kDa
Purification:
SUZ12 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SUZ12 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SUZ12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SUZ12
Additional Names:
SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein
Protein Accession Number:
NP_056170
Protein Gi Number:
197333809
Ncbi Gene Id Number:
23512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1). SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2). It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3,4). SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5).
Background Reference 1:
Kirmizis A, Bartley SM, Kuzmichev A, et al. Silencing of human polycomb target genes is associated with methylation of Histone H3 Lys 27. Genes Dev. 2004; 18:1592-605.
Background Reference 2:
Pasini D, Bracken AP, Hansen JB, et al. The polycomb group protein Suz12 is required for embryonic stem cell differentiation. Mol. Cell. Biol. 2007; 27:3769-79.
Background Reference 3:
Cao R and Zhang Y. SUZ12 is required for both the Histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol. Cell 2004; 15:57-67.
Background Reference 4:
Pasini D, Bracken AP, Jensen MR, et al. SUZ12 is essential for mouse development and for EZH2 Histone methyltransferase activity. EMBO J. 2004; 23:4061-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7701

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369