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46-452 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-452 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SV2A Antibody

CATALOG NUMBER: 46-452

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TVELYPSDKRTTA
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Rat Brain lysates (calculated MW of 82.6kDa according to rat NP_476558.1). Recommended concentration: 0.3-1ug/ml. Primary incuabtion was 1 hour. An additonal band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SV2A
Additional Names:
SV2A, synaptic vesicle glycoprotein 2A, KIAA0736, SV2 , synaptic vesicle glycoprotein 2
Protein Accession Number:
NP_055664.3
Protein Gi Number:
41281524
Ncbi Gene Id Number:
9900
Background Reference 1:
Dong M, Yeh F, Tepp WH, Dean C, Johnson EA, Janz R, Chapman ER. SV2 is the protein receptor for botulinum neurotoxin A. Science. 2006 Apr 28;312(5773):592-6. Epub 2006 Mar 16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-452

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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