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46-454 (0.5ug/ml) staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-454 (2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-454 (0.5ug/ml) staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Swiprosin 1 Antibody

CATALOG NUMBER: 46-454

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CAAFKELQSTFK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Thymus, Human Lymph node and Human Tonsil lysates (calculated MW of 26.7kDa according to NP_077305.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EFHD2
Additional Names:
EFHD2, MGC4342, EF hand domain containing 2, EF-hand domain family, member D2, RP3-467K16.3, SWS1, EF hand domain family, member D2, swiprosin 1
Protein Accession Number:
NP_077305.2
Protein Gi Number:
20149675
Ncbi Gene Id Number:
79180
Background Reference 1:
Vuadens F, Rufer N, Kress A, Corthesy P, Schneider P, Tissot JD. Identification of swiprosin 1 in human lymphocytes. Proteomics. 2004 Aug;4(8):2216-20.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-454

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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