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Western blot analysis of lysed extracts from JK cells using syk (Ab-323).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (Ab-323).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using syk (Ab-323).
Western blot analysis of lysed extracts from JK cells using syk (Ab-323).

syk (Ab 323) Antibody

CATALOG NUMBER: 79-434

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
syk (Ab-323) antibody was raised against a peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total syk protein.
Predicted Molecular Weight:
72 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SYK
Additional Names:
p72-Syk, IKBE, Spleen tyrosine kinase
Protein Accession Number:
NP_001128524.1
Protein Gi Number:
205277435
Ncbi Gene Id Number:
6850
Background:
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C γdependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Background Reference 1:
Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447. Turner, M. et al. (2000) Immunol. Today 21, 148-154. Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-434

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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