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Western Blot at 1:2000 dilution Lane 1: THP-1 whole cell lysates Lane 2: Ramos whole cell lysates Lane 3: mouse spleen lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: THP-1 whole cell lysates Lane 2: Ramos whole cell lysates Lane 3: mouse spleen lysates Lysates/proteins at 20 ug per lane.

SYK Antibody

CATALOG NUMBER: 56-631

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This SYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-305 amino acids from human SYK.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYK
Additional Names:
Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk, SYK
Protein Accession Number:
P43405
Protein Gi Number:
1174527
Ncbi Gene Id Number:
6850
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the family of non-receptortype Tyr protein kinases. This protein is widely expressed inhematopoietic cells and is involved in coupling activatedimmunoreceptors to downstream signaling events that mediate diversecellular responses, including proliferation, differentiation, andphagocytosis. It is thought to be a modulator of epithelial cellgrowth and a potential tumour suppressor in human breastcarcinomas. Alternatively spliced transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Thomas, D.H., et al. Blood 116(14):2570-2578(2010)
Background Reference 2:
Poeck, H., et al. J. Mol. Med. 88(8):745-752(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Pechloff, K., et al. J. Exp. Med. 207(5):1031-1044(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-631

List Size:
400 ul

List Price:

$495.00

Datasheet
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