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Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~78k Synapsin I protein.
Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~78k Synapsin I protein.

Synapsin (phospho Ser9) Antibody

CATALOG NUMBER: XPS-1025

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Immunogen:
Synapsin (Ser9) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser9 of Rat Synapsin I.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody also weakly labels Synapsin II (55k) which has a similar phosphorylation site to that of Ser9 on Synapsin I. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
Synapsin antibody is specific for Synapsin I phosphorylated at Ser9.
Predicted Molecular Weight:
78
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
SYN1
Protein Accession Number:
P17599
Protein Gi Number:
1351166
Ncbi Gene Id Number:
281510
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.
Background Reference 1:
Kao, H.T., Song, H.J., Porton, B., Ming, G.L., Hoh, J., Abraham, M., Czernik, A.J., Pieribone, V.A., Poo, M.M., and Greengard, P., "A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth," Nature Neurosci., 5 (2002) 431 - 437.
Background Reference 2:
Jovanovic, J.N., Sihra, T.S., Nairn, A.C., Hemmings, H.C., Jr., Greengard, P., and Czernik, A.J., "Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals," J. Neurosci., 21 (2001) 7944 - 7953.
Background Reference 3:
Menegon, A., Dunlap, D.D., Castano, F., Benfenati, F., Czernik, A.J., Greengard, P., and Valtorta, F., "Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals," J. Cell Sci., 113 (2000) 3573 - 3582.
Background Reference 4:
Hosaka, M, Hammer, R.E., Sudhof, T.C., "A phospho-switch controls the dynamic association of synapsins with synaptic vesicles," Neuron, 24 (1999) 377-87.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1025

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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