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Western blot analysis of various lysates using Synaptophysin Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Confocal imaging of paraffin-embedded Mouse brain using Synaptophysin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Rat brain using Synaptophysin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
Confocal imaging of frozen sections Mouse brain tissue using Synaptophysin Rabbit mAb followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of various lysates using Synaptophysin Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

Synaptophysin Rabbit Recombinant Antibody (81-388)

CATALOG NUMBER: 81-388

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, WB
Application Note:
WB,1:1000 - 1:2000, IF-P,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse brain,Rat brain
Predicted Molecular Weight:
34kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SYP
Additional Names:
MRX96; MRXSYP; XLID96; Synaptophysin
Protein Accession Number:
P08247
Ncbi Gene Id Number:
6855
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-388

List Size:
100 uL

List Price:

$540.00

Datasheet
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