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Western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).
SYNCI Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYNCI Antibody (N-term)for immunohistochemistry.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).

SYNCI Antibody

CATALOG NUMBER: 55-432

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SYNCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the N-terminal region of human SYNCI.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYNC
Additional Names:
Syncoilin, Syncoilin intermediate filament 1, Syncoilin-1, SYNC, SYNC1
Protein Accession Number:
Q9H7C4
Protein Gi Number:
300669677
Ncbi Gene Id Number:
81493
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the intermediate filamentfamily which contains an N-terminal head domain, followed by acentral coiled-coil region and a short C-terminal tail. The proteinis highly expressed in skeletal and cardiac muscle. The proteinlinks the dystrophin associated protein complex (DAPC) to desminfilaments in muscle and may have a structural role in striatedmuscle. Multiple transcript variants encoding different isoformshave been found for this gene.
Background Reference 1:
Wakayama, Y., et al. Int. J. Neurosci. 120(2):144-149(2010)
Background Reference 2:
Jordanova, A., et al. Nat. Genet. 38(2):197-202(2006)
Background Reference 3:
Brown, S.C., et al. Muscle Nerve 32(6):715-725(2005)
Background Reference 4:
Poon, E., et al. J. Biol. Chem. 277(5):3433-3439(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-432

List Size:
400 ul

List Price:

$495.00

Datasheet
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