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43-488 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-488 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

syndecan 1 / CD138 Antibody

CATALOG NUMBER: 43-488

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-REQEATPRPRETTQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 37kDa band observed in lysates of cell line HepG2 and of Gastrointensinal cancer and Spinal Cord cancer (calculated MW of 32.5kDa according to NP_002988.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3ug/ml.
Specificity:
Reported variants represent identical protein: NP_001006947.1, NP_002988.3.
Predicted Molecular Weight:
Approx 37kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
syndecan 1 / CD138
Additional Names:
SDC1, syndecan 1, CD138, SDC, SYND1, syndecan, CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1, syndecan-1
Protein Accession Number:
NP_002988.3
Protein Gi Number:
229463011
Ncbi Gene Id Number:
6382
Background Reference 1:
Wu YH, Yang CY, Chien WL, Lin KI, Lai MZ.Removal of syndecan-1 promotes TRAIL-induced apoptosis in myeloma cells.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-488

List Size:
0.1 mg

List Price:

$473.00

Datasheet
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