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Western blot analysis of SYNPO2L in human thymus tissue lysate with SYNPO2L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of SYNPO2L in mouse skeletal muscle tissue with SYNPO2L antibody at 20 μg/mL.
Western blot analysis of SYNPO2L in human thymus tissue lysate with SYNPO2L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SYNPO2L Antibody

CATALOG NUMBER: 6053

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SYNPO2L antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SYNPO2L.
The immunogen is located within amino acids 140 - 190 of SYNPO2L.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SYNPO2L antibody can be used for detection of SYNPO2L by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Purification:
SYNPO2L Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SYNPO2L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SYNPO2L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYNPO2L
Additional Names:
SYNPO2L Antibody: Synaptopodin 2-like protein
Protein Accession Number:
BAD37139
Protein Gi Number:
51534920
Ncbi Gene Id Number:
79933
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SYNPO2L Antibody: SYNPO2L was initially identified as a novel heart-enriched gene that encodes a cytoskeletal protein highly expressed in the Z-disc of heart and skeletal muscle, associates with actin and interacts with a-actinin. It is a member of the synaptopodin family, sharing greatest homology with Synaptopodin 2. Recent studies have shown that SYNPO2L, while primarily localized to the sarcomere, can also translocate to the nucleus. A knockdown of SYNPO2L in zebrafish resulted in aberrant cardiac and skeletal muscle development and function, suggesting that it is a critical component of the sarcomere and plays an important role in muscle development.
Background Reference 1:
Beqqali A, Kloots J, Ward-van Oostward D, et al. Genome-wide transcriptional profiling of human embryonic stem cells differentiating to cardiomyocytes. Stem Cells2006; 24:1956-67.
Background Reference 2:
Beqqali A, Manshouwer-Kloots J, Moneiro R, et al. CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. J. Cell Sci.2010; 123:1141-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6053

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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